From my numerous observations, I conclude that these tubercle bacilli occur in all tuberculous disorders, and that they are distinguishable from all other microorganisms.

However, on many occasions, I examined normal blood and normal tissues and there was no possibility of overlooking bacteria or confusing them with granular masses of equal size. I never found organisms. Thus, I conclude that bacteria do not occur in healthy human or animal tissues.

If my efforts have led to greater success than usual, this is due, I believe, to the fact that during my wanderings in the field of medicine, I have strayed onto paths where the gold was still lying by the wayside. It takes a little luck to be able to distinguish gold from dross, but that is all.

In the first papers concerning the aetiology of tuberculosis I have already indicated the dangers arising from the spread of the bacilli-containing excretions of consumptives, and have urged moreover that prophylactic measures should be taken against the contagious disease. But my words have been unheeded. It was still too early, and because of this they still could not meet with full understanding. It shared the fate of so many similar cases in medicine, where a long time has also been necessary before old prejudices were overcome and the new facts were acknowledged to be correct by the physicians.

It is safe to say that the little pamphlet which was left to find its way through the slow mails to the English scientist outweighed in importance and interest for the human race all the press dispatches which have been flashed under the channel since the delivery of the address—March 24. The rapid growth of the Continental capitals, the movements of princely noodles and fat, vulgar Duchesses, the debates in the Servian Skupschina, and the progress or receding of sundry royal gouts are given to the wings of lightening; a lumbering mail-coach is swift enough for the news of one of the great scientific discoveries of the age. Similarly, the gifted gentlemen who daily sift out for the American public the pith and kernel of the Old World's news; leave Dr. KOCH and his bacilli to chance it in the ocean mails, while they challenge the admiration of every gambler and jockey in this Republic by the fullness and accuracy of their cable reports of horse-races.

Our studies have shown that all cases of typhoid of this type have arisen by contact, that is, carried directly from one person to another. There was no trace of a connection to drinking water.

The facts obtained in this study may possibly be sufficient proof of the causal relationship, that only the most sceptical can raise the objection that the discovered microorganism is not the cause but only an accompaniment of the disease... It is necessary to obtain a perfect proof to satisfy oneself that the parasite and the disease are ... actually causally related, and that the parasite is the... direct cause of the disease. This can only be done by completely separating the parasite from the diseased organism [and] introducing the isolated parasite into healthy organisms and induce the disease anew with all its characteristic symptoms and properties.

The pure culture is the foundation for all research on infectious disease.

To prove that tuberculosis is caused by the invasion of bacilli, and that it is a parasitic disease primarily caused by the growth and multiplication of bacilli, it is necessary to isolate the bacilli from the body, to grow them in pure culture until they are freed from every disease product of the animal organism, and, by introducing isolated bacilli into animals, to reproduce the same morbid condition that is known to follow from inoculation with spontaneously developed tuberculous material.